high-speed microarray centrifuge  (TeleChem International)

Journal: Acta Obstetricia et Gynecologica Scandinavica

Article Title: A novel multiple marker microarray analyzer and methodology to predict major obstetric syndromes using surface markers of circulating extracellular vesicles from maternal plasma

doi: 10.1111/aogs.15020

Figure Lengend Snippet: Principle of analysis of the extracellular vesicle (EV) Array. A customized antibody microarray is generated onto glass slides. EVs from unprocessed plasma or UC enriched EVs are added and EVs are captured during incubation. Step 2 is either performed using a cocktail of anti‐CD9, CD63 and CD81 to detect the total amount of EVs or by using anti‐PP13 to detect only placenta‐derived EVs. After Step 2 fluorescent streptavidin is added prior to scanning.

Article Snippet: Then, the complexes were marked with cyanine 5–labeled streptavidin (Life Technologies, Carlsbad, CA, USA), and diluted 1:3000 in wash buffer for 30 min. Access were washed out in wash buffer followed by MilliQ water, and dried using a Microarray High‐Speed Centrifuge (ArrayIt Corporation, San Francisco, CA, USA).

Techniques: Microarray, Generated, Incubation, Derivative Assay

Journal: BMC Bioinformatics

Article Title: Improvement in the Reproducibility and Accuracy of DNA Microarray Quantification by Optimizing Hybridization Conditions

doi: 10.1186/1471-2105-7-S2-S17

Figure Lengend Snippet: Comparison of transcript expression in MTRRMs (Mix1/Mix2) by qRT-PCR and microarray.

Article Snippet: The slides were spun dry in a microarray high speed centrifuge (TeleChem International, Sunnyvale, CA).

Techniques: Expressing, Microarray

Journal: BMC Genomics

Article Title: RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae

doi: 10.1186/1471-2164-14-607

Figure Lengend Snippet: Microarray analyses of Verticillium dahliae genes induced in a wild type strain and repressed in a hydrophobin mutant strain ( vdh1 )

Article Snippet: Following pre-hybridization slides were rinsed thoroughly with sterile distilled, deionized water (DDW), and dried using an ArrayIt microarray high-speed centrifuge (TeleChem International, Sunnyvale, CA).

Techniques: Microarray, Mutagenesis, Expressing, Binding Assay